Bin2 phosphorylation mutant
WebBIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes … WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced …
Bin2 phosphorylation mutant
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WebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated … WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes the phosphorylation of BES1 in far-red light. BIN2 acts genetically downstream from PHYA to regulate photomorphogenesis under far-red light.
WebMay 14, 2024 · We thus tested whether BIN2 mediates the phosphorylation of BZR1 on high sugar medium. It is reported that BIN2 interacts directly with BZR1 through a 12-amino … WebBIN2 integrates multiple signaling pathways by phosphorylating a range of proteins including the components in light signaling. For example, PIF3, PIF4, and PIF5 can be phosphorylated by BIN2 and then degraded via the 26S proteasome pathway to control transcription and hypocotyl growth ( Bernardo-García et al., 2014; Ling et al., 2024 ).
WebBIN2 phosphorylation of RD26 is required for RD26 transcriptional activation on drought-responsive genes. RD26 overexpression suppressed the brassinazole (BRZ) insensitivity of BIN2 triple mutant bin2 bil1 bil2, … WebNov 14, 2024 · The BIN2–POLAR–BASL complex migrates to the cell cortex and polarizes before the ACD, thereby relieving the BIN2 inhibition on SPCH in the nucleus and attenuating the MAPK signalling in the...
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To further identify the mechanism by which HY5 regulated BIN2 in the light to destabilize BZR1, we first checked whether HY5 regulated the mRNA and/or protein levels of BIN2. As shown in Supplementary Fig. 5, both the mRNA and protein levels of BIN2 were similar in hy5 and WT, suggesting that HY5 does not … See more To investigate the cross-talk between light and BR signaling, we performed yeast two-hybrid analyses and found that BIN2, the key negative … See more Next, we investigated whether HY5 could genetically interact with BIN2 by crossing hy5 into bin2-1, a gain-of-function mutant of BIN228. We found that the hypocotyls of double mutant … See more To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. As shown in Fig. 5a, changing HY5 Leu137 (L137) to Asp (named HY5-M) … See more Previous reports have suggested that HY5 and BIN2 have similar effects on BZR1 protein accumulation20,27. To gain further insight into their … See more song all i ask of you phantom of the operaWebAug 1, 2024 · BR-INSENSTIVE 2 (BIN2), a crucial negative regulator of brassinosteroid (BR) signaling, is induced by ABA and phosphorylates SnRK2s to enhance ABA signaling [5]. PP2Cs, such as ABI1 and ABI2, function as negative regulators of both BIN2 and SnRK2s, and thereby form a negative feedback regulatory loop in ABA signaling [12]. song all i ask that remember me as loving youWebJul 22, 2009 · Plant Materials and Growth Conditions. Arabidopsis thaliana ecotype Columbia was used as WT control. BR-deficient mutant det2-1 and BR-insensitive mutant bin2-1 were obtained from Dr. Zhiyong Wang and Dr. Jun Zhao. WT and det2-1 seeds were surface-sterilized in 10% sodium hypochlorite and rinsed five times in sterile water. The … small dog sweater pattern crochetWebJun 6, 2024 · Considering the BIN2 inhibitor bikinin greatly increased SA-induced gene expression (Figure 1 D–1F and Supplemental Figure 1 B–1D), we examined the functional involvement of BIN2 in SA signaling.The bin2-1 gain-of-function mutant displayed reduced sensitivity to SA in a bacterial growth assay (Figure 2 B). Similarly, the inhibitory effect of … song all i am is yours by phil wickhamWebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated protein of TOR 1B), a substrate-recruiting subunit in the TOR complex, at a conserved serine residue within a typical BIN2 phosphorylation motif. song all hindiWebMay 25, 2024 · The disease symptoms caused by V. dahliae were more severe in the gain-of-function mutant bin2-1 seedlings compared with the control plants Col-0. The loss-of … song all i ask of you susan boyleWebNov 19, 2012 · BIN2 is a negative regulator in the BR pathway, which phosphorylates and inhibits BES1 and BZR1 (4, 48–50). BIN2 belongs to glycogen synthase kinase 3 … song all i ask of you by barbra streisand